Primer Walk

Primer walking is a method of for sequencing DNA fragments between 1.3 and 7 kb. . The DNA of interest may be either a plasmid insert or a PCR product. The initial sequencing will be performed from each end using either universal primers or designed primers. In order to completely sequence the region of interest BioScript will design and synthesize primers as necessary to obtain coherent sequence information. We offer following services.

SSPW – Single Stranded Primer Walk, DSPW – Double Stranded Primer Walk.