Peptide Mass Fingerprinting

Peptide mass fingerprinting is an analytical technique for protein identification the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. These masses are then compared to a reference database. Through Bioinformatics analysis translate the known genome of the organism into proteins, then theoretically cut the proteins into peptides, and calculate the absolute masses of the peptides from each protein. They then compare the masses of the peptides of the unknown protein to the theoretical peptide masses of each protein encoded in the genome to know the protein sequence.