Whole Genome Sequencing

BioScript offers whole genome sequencing services with all three Next Gen Instruments. It includes, Fragmentation by nebulization of the genomic DNA, Preparation of a non-cloned single stranded shotgun library (sstDNA) and clonal amplification by emulsion PCR (emPCR),  Ultra-high throughput sequencing with GS FLX Titanium-series chemistry up to 20 fold coverage, Assembly with appropriate assembly software, Generation and sequencing of non-cloned paired end libraries, Assembly of the paired ends and scaffolding the contigs, Closing of gaps by designing primer pairs on adjacent contig ends and sequencing PCR amplicons of the genomic region of interest,  Bioinformatics analysis such as ORF calling and annotation, Subsequent gene synthesis services can be performed to generate clones for specific genes of interest.